iscript cdna 58 synthesis kit Search Results


smart seq v4  (TaKaRa)
98
TaKaRa smart seq v4
Smart Seq V4, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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purelink genomic dna 58 mini kit  (Thermo Fisher)
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Thermo Fisher purelink genomic dna 58 mini kit
Purelink Genomic Dna 58 Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscript cdna 58 synthesis kit  (Bio-Rad)
99
Bio-Rad iscript cdna 58 synthesis kit
Iscript Cdna 58 Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscript cdna 58 synthesis kit - by Bioz Stars, 2026-03
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reactive oxygen species assay kit  (Beyotime)
90
Beyotime reactive oxygen species assay kit
Reactive Oxygen Species Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromium genome reagent kit v2 (cat# 120257/58/61/62)  (10X Genomics)
90
10X Genomics chromium genome reagent kit v2 (cat# 120257/58/61/62)
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7 5 1 58 0 01 dneasy blood tissue kit qiagen silica membrane column spin  (Qiagen)
99
Qiagen 7 5 1 58 0 01 dneasy blood tissue kit qiagen silica membrane column spin
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58/38 race kit  (Boehringer Mannheim)
90
Boehringer Mannheim 58/38 race kit
58/38 Race Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enzymatic kit  (Pointe Scientific)
90
Pointe Scientific enzymatic kit
Enzymatic Kit, supplied by Pointe Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bhb kit, cat. no. h7587–58  (Pointe Scientific)
90
Pointe Scientific bhb kit, cat. no. h7587–58
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taqman microrna reverse transcription kit  (Thermo Fisher)
90
Thermo Fisher taqman microrna reverse transcription kit
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orlistat  (Millipore)
90
Millipore orlistat
Biological functions of lipid synthesis pathway in Ewing sarcoma cells. ( A, B ) Silencing of each of three CRC TFs decreased the expression of lipid biosynthetic enzymes at both the mRNA (A) and protein levels (B) in Ewing sarcoma cell lines. ( C ) IGV plots showing FASN and SCD promoters which were trio-occupied by three CRC TFs. ( D ) Silencing of FASN and SCD by siRNAs ( E ) decreased Ewing sarcoma cell proliferation. ( F ) In vitro MTT proliferation assay of indicated cells in the presence of different doses of <t>orlistat</t> (FASN inhibitor). ( G ) Orlistat treatment suppressed xenograft growth in vivo . Growth curves of both groups are shown. (H, I) Silencing of each CRC TFs decreased SPTLC1 expression at both the mRNA ( H ) and protein levels ( I ) in Ewing sarcoma cell lines. (J–L) Silencing of SPTLC1 by siRNA ( J ) decreased colony growth ( K ) and cell proliferation ( L ) in Ewing sarcoma cell lines. (M, N) SPTLC <t>inhibitor</t> <t>(Myriocin)</t> decreased cell proliferation ( M ) and colony growth ( N ) in indicated cells. NC, non-targeting control siRNA. Data are presented as mean ± s.d. of three replicates. Two-way ANOVA Dunnett's multiple comparisons test are performed. (A), (D), (E), (H), (J), (K), (L) and (N) are n = 3, (G) is n = 6. * P < 0.5, ** P < 0.01; *** P < 0.001.
Orlistat, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nanovue spectrophotometer  (GE Healthcare)
86
GE Healthcare nanovue spectrophotometer
Biological functions of lipid synthesis pathway in Ewing sarcoma cells. ( A, B ) Silencing of each of three CRC TFs decreased the expression of lipid biosynthetic enzymes at both the mRNA (A) and protein levels (B) in Ewing sarcoma cell lines. ( C ) IGV plots showing FASN and SCD promoters which were trio-occupied by three CRC TFs. ( D ) Silencing of FASN and SCD by siRNAs ( E ) decreased Ewing sarcoma cell proliferation. ( F ) In vitro MTT proliferation assay of indicated cells in the presence of different doses of <t>orlistat</t> (FASN inhibitor). ( G ) Orlistat treatment suppressed xenograft growth in vivo . Growth curves of both groups are shown. (H, I) Silencing of each CRC TFs decreased SPTLC1 expression at both the mRNA ( H ) and protein levels ( I ) in Ewing sarcoma cell lines. (J–L) Silencing of SPTLC1 by siRNA ( J ) decreased colony growth ( K ) and cell proliferation ( L ) in Ewing sarcoma cell lines. (M, N) SPTLC <t>inhibitor</t> <t>(Myriocin)</t> decreased cell proliferation ( M ) and colony growth ( N ) in indicated cells. NC, non-targeting control siRNA. Data are presented as mean ± s.d. of three replicates. Two-way ANOVA Dunnett's multiple comparisons test are performed. (A), (D), (E), (H), (J), (K), (L) and (N) are n = 3, (G) is n = 6. * P < 0.5, ** P < 0.01; *** P < 0.001.
Nanovue Spectrophotometer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Biological functions of lipid synthesis pathway in Ewing sarcoma cells. ( A, B ) Silencing of each of three CRC TFs decreased the expression of lipid biosynthetic enzymes at both the mRNA (A) and protein levels (B) in Ewing sarcoma cell lines. ( C ) IGV plots showing FASN and SCD promoters which were trio-occupied by three CRC TFs. ( D ) Silencing of FASN and SCD by siRNAs ( E ) decreased Ewing sarcoma cell proliferation. ( F ) In vitro MTT proliferation assay of indicated cells in the presence of different doses of orlistat (FASN inhibitor). ( G ) Orlistat treatment suppressed xenograft growth in vivo . Growth curves of both groups are shown. (H, I) Silencing of each CRC TFs decreased SPTLC1 expression at both the mRNA ( H ) and protein levels ( I ) in Ewing sarcoma cell lines. (J–L) Silencing of SPTLC1 by siRNA ( J ) decreased colony growth ( K ) and cell proliferation ( L ) in Ewing sarcoma cell lines. (M, N) SPTLC inhibitor (Myriocin) decreased cell proliferation ( M ) and colony growth ( N ) in indicated cells. NC, non-targeting control siRNA. Data are presented as mean ± s.d. of three replicates. Two-way ANOVA Dunnett's multiple comparisons test are performed. (A), (D), (E), (H), (J), (K), (L) and (N) are n = 3, (G) is n = 6. * P < 0.5, ** P < 0.01; *** P < 0.001.

Journal: Nucleic Acids Research

Article Title: EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma

doi: 10.1093/nar/gkaa901

Figure Lengend Snippet: Biological functions of lipid synthesis pathway in Ewing sarcoma cells. ( A, B ) Silencing of each of three CRC TFs decreased the expression of lipid biosynthetic enzymes at both the mRNA (A) and protein levels (B) in Ewing sarcoma cell lines. ( C ) IGV plots showing FASN and SCD promoters which were trio-occupied by three CRC TFs. ( D ) Silencing of FASN and SCD by siRNAs ( E ) decreased Ewing sarcoma cell proliferation. ( F ) In vitro MTT proliferation assay of indicated cells in the presence of different doses of orlistat (FASN inhibitor). ( G ) Orlistat treatment suppressed xenograft growth in vivo . Growth curves of both groups are shown. (H, I) Silencing of each CRC TFs decreased SPTLC1 expression at both the mRNA ( H ) and protein levels ( I ) in Ewing sarcoma cell lines. (J–L) Silencing of SPTLC1 by siRNA ( J ) decreased colony growth ( K ) and cell proliferation ( L ) in Ewing sarcoma cell lines. (M, N) SPTLC inhibitor (Myriocin) decreased cell proliferation ( M ) and colony growth ( N ) in indicated cells. NC, non-targeting control siRNA. Data are presented as mean ± s.d. of three replicates. Two-way ANOVA Dunnett's multiple comparisons test are performed. (A), (D), (E), (H), (J), (K), (L) and (N) are n = 3, (G) is n = 6. * P < 0.5, ** P < 0.01; *** P < 0.001.

Article Snippet: Reagents and kits included: Orlistat (Sigma-Aldrich, 96829-58-2-, Myriocin, (Sigma-Aldrich, 35891-70-4-, Propidium iodide (Sigma-Aldrich, 25535-16-4-, FITC Annexin V Apoptosis Detection Kit (BD Biosciences), Dual-Luciferase Reporter Assay System (Promega), BioT transfection reagent (Bioland Scientific), Lipofectamine RNAiMAX transfection reagent (Invitrogen), and siRNA pools targeting KLF15 , TCF4 , NKX2-2 , FLI-1 , FASN , SCD , RREB1 and SPTLC1 (Dharmacon). siRNA sequences are provided in .

Techniques: Expressing, In Vitro, Proliferation Assay, In Vivo